We previously constructed a synthetic E. coli genetic network which produces oscillations in protein levels. However, when E. coli cells divide, they quickly lose synchrony with their progeny within 2-3 cell divisions producing a wide variability in oscillation phase across a population. I am studying an intracellular quorum sensing circuit that will enable cells to communicate their phases and allow them to synchronize their oscillatory behavior. Theoretical work has previously shown a design in which cells could synchronize via the luxIR system from Vibrio Fischeri. Molecular cloning techniques, microfluidics, and modelling are used to study the dynamics of synchronization for a population of cells.

Abstract Type
poster
Primary Author
Tal Danino
University
University of California, San Diego
Abstract Title
Synchronization of an E. coli genetic oscillator
Abstract Author(s)
Tal Danino, Jesse Stricker, Lev Tsimring, Jeff Hasty
Username
danino
Fellowship Year
2006
First Name
Tal
Last Name
Danino
Program
CSGF
Area of Study
Bioengineering
Poster Group
Group 2
Poster Number
10